Table 3.
Immunohistochemical analysis of specimens (cadaveric vincula, vincula from patients’ biopsies, sural nerve, extensor retinaculum)
| specimen | S100 | p 75 | MBP | PGP 9.5 | vimentin | NF |
|---|---|---|---|---|---|---|
| Cadaveric peroneal vincula | ++ | + | ++ | ++ | ++ | ++ |
| Vincula from patients’ biopsies | ++ | / | / | / | / | / |
| Sural nerve | ++ | ++ | ++ | / | / | / |
| Inferior extensor retinaculum | ++ | ++ | ++ | + | ++ | / |
S-110 = S-100 protein is a family of low molecular weight proteins found in vertebrates, characterized by two calcium binding sites of the helix-loop-helix (“EF-hand type”) conformation; the name is derived from the fact that the protein is 100 % soluble in ammonium sulfate at neutral pH
p75. = low-affinity neurotrophin receptor, NGFR, nerve growth factor receptor, CD271
MBP = myelin basic protein
PGP 9.5 = pan-neuronal marker protein gene product
NF = neurofilament
Vimentin = type III intermediate filament (IF) protein that is expressed in all mesenchymal cells; here it is used as an undifferentiated marker for both nervous and vascular structures
++ = strong positive staining at immunohistochemistry
+ = weak positive staining at immunohistochemistry
/ = staining not performed