Fig. 1.

Nucleotide and deduced amino acid sequences of PtHSP60 (GenBank accession JN628037). The nucleotide sequence is numbered from the 5′end, and the single letter aa code is shown below the corresponding codon. Nucleotide and deduced amino acid residues are numbered on the left. The start code ATG and the termination code are shown underlined. The classical mt-HSP60 signature motifs were double underlined. The 1,734 bp (577 amino acids) sequence contains a presequence of 27 amino acids at the N terminus that is required for import into the mitochondria (underlined). The ATP-binding motif and a typical GGM repeat motif at the C terminus are boxed. The locations of three pairs of primers (PtHSP60-F1/PtHSP60-R1, PtHSP60-F2/PtHSP60-R2, PtHSP60-F3/PtHSP60-R3) designed for PCR used in our study are indicated by arrow and detailed sequences for those primers are indicated by light grey