Fig. 5.

a RT-PCR analysis of the SOD-1, SOD-2, and HO-1 expression by the Nrf2-MSCs. Expression of these genes was up-regulated in two Nrf2 overexpressing clones. M 100 bp DNA marker. b Quantification of the bands was analyzed by UVIdoc Gel Documentation System (Avebury House 36a Union Lane Cambridge CB4 1QB-uk). c Total SOD and HO-1 activities were compared between test (V-Nrf2) and control (V) groups. Results showed significant enhancement of activities of these proteins following Nrf2 overexpression (mean±SD; ***p < 0.001; three independent experiments were carried out)