Fig. 2.

Effect dnHSF1 and HSPA1A or DNAJB1 on refolding capacities of different organelles. Relative luciferase activity of a Cyt-superluc-eGFP in HEK-cDNA5 and HEK-dnHSF1 cells; b Nuc-superluc-eGFP in HEK-cDNA5 and HEK-dnHSF1 cells; c ER-superluc-eGFP in HEK-cDNA5 and HEK-dnHSF1 cells; d Per-superluc-eGFP in HEK-cDNA5-Per-superluc-eGFP or HEK-dnHSF1-Per-superluc-eGFP cells. Cells were co-transfected with the luciferase reporter gene and expression constructs for HSPA1A and/or DNAJB1 and/or empty vector as indicated. Per-superluc-eGFP cells were transfected only with the expression constructs. Expression levels of exogenously expressed proteins are shown in Fig. S5. Cells were harvested after heat shock at the times indicated, and the luciferase activity was measured. Relative luciferase activities were calculated as detailed in the legend to Fig. 1. The results are the average of four independent transfections (standard deviations are indicated by error bars)