Fig. 3.

HSF1 dependent thermotolerance in different organelles. Thermotolerance in different cellular compartments. a Cyt-superluc-eGFP in HEK-cDNA5 and HEK-dnHSF1 cells; b Nuc-superluc-eGFP in HEK-cDNA5 and HEK-dnHSF1 cells; c ER-superluc-eGFP in HEK-cDNA5 and HEK-dnHSF1 cells; d Per-superluc-eGFP in HEK-cDNA5-Per-superluc-eGFP or HEK-dnHSF1-Per-superluc-eGFP cells. Cells were either given a pre-heat shock at 45°C for 30 min or left untreated. Thirteen hours later, a second heat shock of 45°C for 30 min was applied. Cells were allowed to recover after heat shock for 1 h. Relative luciferase activities were calculated as detailed in the legend to Fig. 1. The results are the average of four independent transfections (standard deviations are indicated by error bars)