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FTIR absorption in the Amide I region is shown for the WT and V257STOP forms both with and without 10 mM DTT at varying temperature. Panels A and B show data collected on the WT form in the absence (A) and presence (B) of DTT. Panels C and D show data collected on the V257STOP mutant form in the absence (C) and presence (D) of DTT. The data shown was collected at 10 °C (red), 25°C (blue), 40 °C (green), 55 °C (purple), 70 °C (light blue) and 90 °C (pink).
