Table 3.
Primers/probes used for real-time RT-PCR (TaqMan® Gene Expression Assays, Applied Biosystems)
| Gene | Primer sequence number | Base pairs |
|---|---|---|
| Aggrecan | Hs00202971_m1 | 93 |
| Collagen-I | Hs00164004_m1 | 66 |
| Collagen-II | Hs00264051_m1 | 124 |
| Interleukin-6 (IL-6) | Hs00174131_m1 | 95 |
| Interleukin-8 (IL-8) | Hs00174103_m1 | 101 |
| Matrixmetalloproteinase-1 (MMP1) | Hs00233958_m1 | 133 |
| Matrixmetalloproteinase-2 (MMP2) | Hs00174131_m1 | 96 |
| Matrixmetalloproteinase-3 (MMP3) | Hs01548724_m1 | 98 |
| Matrixmetalloproteinase-13 (MMP13) | Hs00233992_m1 | 91 |
| Prostaglandin E2 Synthase (PGE2S) | Hs00228159_m1 | 66 |
| Tata Box binding protein (TBP) | Hs00427620_m1 | 91 |
| Toll-like receptor 2 (TLR2) | Hs00152932_m1 | 80 |
| Toll-like receptor 4 (TLR4) | Hs00152939_m1 | 89 |
| Tumor necrosis factor α (TNF-α) | Hs00174128_m1 | 80 |