Table 4.

Effects of IL-1β stimulation on mRNA levels of candidate genes after 6, 18 and 30 h, indicated as fold change relative to no treatment (mean, SEM, P values)

Gene	Time point (h)	Mean fold change	SEM	P value
Aggrecan	6	−1.4	0.3	0.001
	18	−1.8	0.5	0.001
	30	−2.7	0.5	<0.0001
Collagen-I	6	−1.0	0.2	0.001
	18	−1.3	0.5	0.015
	30	−2.1	0.2	<0.0001
Collagen-II	6	−2.1	0.2	<0.0001
	18	−8.9	3.2	<0.0001
	30	−7.2	1.1	<0.0001
IL-6	6	432.7	74.3	<0.0001
	18	6236.1	2601.3	<0.0001
	30	7350.5	2557.3	<0.0001
IL-8	6	558.4	175.2	<0.0001
	18	1102.5	291.3	<0.0001
	30	1983.8	649.3	<0.0001
MMP1	6	449.1	163.9	<0.0001
	18	1576.5	470.9	<0.001
	30	2032.6	631.9	<0.0001
MMP2	6	0.7	0.3	0.419
	18	2.1	0.5	<0.0001
	30	3.4	0.3	<0.0001
MMP3	6	327.7	124.9	<0.0001
	18	1143.5	240.7	<0.0001
	30	1768.8	636.0	<0.0001
MMP13	6	79.1	20.6	<0.0001
	18	114.6	30.2	<0.0001
	30	297.3	140.0	<0.0001
TNF-α	6	78.5	16.4	<0.0001
	18	41.1	14.4	<0.0001
	30	46.0	11.3	<0.0001
TLR2	6	16.5	1.9	<0.0001
	18	13.5	1.2	<0.0001
	30	13.4	2.1	<0.0001

Data was obtained by real-time RT-PCR ( method) (n = 5). PGE2S and TLR4 were not significantly regulated by IL-1β stimulation (values not shown)