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. Author manuscript; available in PMC: 2013 Dec 5.
Published in final edited form as: J Am Chem Soc. 2012 Nov 26;134(48):19548–19551. doi: 10.1021/ja308908p

Figure 1.

Figure 1

Synthesis of uH2A: a) Synthesis scheme: i) Ligation of peptide 1 to ubiquitin(1-75) α-thioester 2, forming the branched protein 3. ii) Deprotection of 3 by methoxylamine treatment. iii) Formation of 6 by ligation of H2A(1-113) α-thioester 5 to 4. iv) Final desulfurization to form uH2A, 7. R: C6H4CH2COOH. b) RP-HPLC data for the ligation between 1 and 2. c) RP-HPLC data for the ligation between 4 and 5. d-e) RP-HPLC and ESI-MS data for uH2A, 7, (M+H)+ = 22511.8 Da, [(M+H)+expected = 22512.0 Da]