Fig 2.

Nutlin-3 can reactivate p53 in cancer cell lines with overexpressed Mdm2 when activation by etoposide is compromised. Quantitative reverse transcription–polymerase chain reaction was used to detect fold induction of the mdm2, waf1, gadd45, puma, and pig3 transcripts in K562 (panel A), A875 and CCF-STTG-1 (panel B), SJSA-1 and MCF-7 (panel C), and H460 and ML-1 (panel D) cells. Exponentially growing cells were either left untreated (white bars), treated with 8 µM etoposide (50 µM for MCF-7 cells) (black bars), or 10 µM Nutlin-3 (gray bars), for 24 hours. All results were normalized to untreated samples and gapdh values. Results are representative of three independent experiments, and error bars indicate standard deviation.