. Author manuscript; available in PMC: 2013 Nov 27.

Published in final edited form as: Biochemistry. 2012 Nov 13;51(47):9581–9591. doi: 10.1021/bi301132k

Table 3.

Comparison of the wild-type GCN4-p1 coiled-coil trimer structure to known trimer-forming mutants.

	GCN4p1 mutant^a
	wt	N₁₆→S	N₁₆→T	N₁₆→Q	N₁₆→X
C_α rmsd to wt (Å)	n/a	0.80	0.55	0.63	0.67
Superhelix parameter
radius (Å)	6.3	6.4	6.3	6.5	6.5
rise per residue (Å)	1.44	1.47	1.44	1.45	1.46
pitch (Å)	130	153	139	140	143

wt is wild-type trimer structure reported here; other structures were published previously (Asn₁₆→Ser, PDB 1IJ3; Asn₁₆→Thr, PDB 1IJ2; Asn₁₆→Gln, PDB 1ZIM; Asn₁₆→Abu, PDB 1ZIJ); Abu = (S)-2-aminobutyric acid;

rmsd values calculated from overlay to wild-type using C_α atoms in residues 1–30.