Skip to main content
. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Pain. 2013 Jan;154(1):59–70. doi: 10.1016/j.pain.2012.09.002

Figure 2.

Figure 2

A: Real-time RT-PCR analysis of differentially expressed miR-181a in spinal dorsal horns (L6-S1) from rats with neonatal cystitis. Two different treatment protocols were followed and tissues were examined on P60 from unchallenge protocol and on P30 from re-challenge protocol. The ordinate value 2−ΔCT corresponds to the miRNA expression relative to endogenous control U87 and represented as mean ±SD (n=4/group). Asterisks indicate significant difference at p value of <0.01(*) and <0.001(**). B: GABAAα−1 3′UTR interaction with miR-181a in HEK293. The percentage reduction in normalized firefly activity against Renilla luciferase activity for cells transfected with Pre-miR-181a was evaluated by defining the normalized firefly luciferase activity for cells transfected with Pre- miR- negative control as 100%. The data represented the normalized luciferase activity of three different sets of transfections.