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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Neurooncol. 2012 Oct 3;110(3):349–357. doi: 10.1007/s11060-012-0979-0

Figure 3.

Figure 3

KU-55933 inhibits ATM-mediated phosphorylation events. (A) Cells treated with indicated concentrations of TMZ or radiation in combination with or without KU-55933, were harvested and processed for western blot analysis of phospho- and total levels of ATM at 24 and 72 hours (* indicates KU-55933 was added a second time to the media 48 hours after initial treatment). (B–C) Cells treated with 30 microM TMZ alone or in combination with 10 microM KU-55933 were harvested at 72 hours and phospho- and total levels of Chk1 and Chk2 were analyzed by western blot. Representative results from three independent experiments are presented where paired parental and TMZ resistant lines were run on the same gel but intermediate non-relevant lanes were cropped out of the image.