Figure 4. Development of a Nano-lantern-based luminescent cAMP indicator.

(a) Schematic of the domain structure of Nano-lantern (cAMP). (b) Relative brightness of recombinant Nano-lantern and Nano-lantern (cAMP) with or without cAMP. These measurements were performed at least in triplicate, and the averaged data and s.d. are shown. (c) A cAMP image of ~100,000 D. discoideum cells expressing Nano-lantern (cAMP1.6) during aggregative morphogenesis. Scale bar, 1 mm. (d) Space and time plot along the yellow lines shown in (c) for 60 min. (e) Time course of the luminescence intensity change at the ROIs indicated in (c). These are typical data from five measurements.