Figure 5. SRM assay transportability to related species.

All SRM assays were developed on basis of the GAS strain SF370 genome. The degree of SRM assay transportability within selected species was determined phylogeny clustering of respective rpoB gene and mapping medium- and high-scoring SRM assays on to respective genome. (a) Transportability for the SRM assays across 75 genomes within the Firmicutes phylum. (b) Average ORF genome coverage of high- and medium-scoring SRM assays within taxonomic ranks. Boxes extend from the 25^th to 75^th percentiles and error bars represent minimum to maximum values. (c) View of SRM assay transportability for 13 GAS genomes deposited in the public domain.