Table 3.

Growth factor candidate substrates for meprin α and β identified by TAILS

Vascular endothelial growth factor A (VEGF-A) and interleukin 18 (IL-18) are known substrates detected by TAILS as substrates within the class of growth factors; the others are novel substrate candidates. Proteins in italic have been further validated in vitro (Fig. 6). Sequences are given in the one letter codes. Arrows indicate the cleavage sites analyzed by MS/MS for meprin α (α) and meprin β (β), Numbers display position of amino acid in full-length protein. Confirmed cleavage sites in the protein and identified in more than one secretome (biological replicate) is indicated in bold.

BMP-7 bone morphogenic protein 7, DKK-1 Dickkopf-related protein 1, FGF-19 fibroblast growth factor, IGFBP-3 insulin growth factor binding protein 3, IGF insulin-like growth factor 1, MMP matrix metalloproteases, TIMP tissue inhibitor of metalloproteases.