Figure 6.

Northern blot analysis of RcdA target genes for wild-type and rcdA mutant. Wild-type Escherichia coli K-12 BW25113 and its rcdA mutant were grown in either LB at 37°C or YESCA at 28°C. Total RNA was prepared at both exponential phase and stationary phase, and immediately subjected to Northern blot analysis under the standard conditions (for details see Experimental Procedures). DIG-labeled hybridization probes are shown on left side of each panel. The amounts of total RNA analyzed were examined by measuring the intensity of ribosomal RNAs.