Arf6 and Arfip mediate ethanol resistance in the same genetic pathway. A, Mutations in Arfip do not increase the ethanol sensitivity of Arf6− mutant flies (NS, p = 0.30, n = 9–14 per genotype). B, To ascertain that this was not attributable to a floor effect of maximal ethanol sensitivity, the ethanol/air flow was reduced to 20:130, which does not sedate wild-type flies (gray bar). Introducing the unrelated ethanol-sensitive cheapdate allele of the Amnesiac gene (dotted bars), but not Arfip12, further decreased the ethanol sensitivity of Arf6 mutant flies (*p < 0.05, n = 5 per genotype). Arf6−, Arf6P2/Gal4.