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. Author manuscript; available in PMC: 2013 Jan 3.
Published in final edited form as: Biochemistry. 2009 Oct 27;48(42):10014–10019. doi: 10.1021/bi901160f

Figure 2.

Figure 2

SH3GK activation screen. (a) SH3GK mutations identified that allow binding to GukH in yeast two-hybrid survival screening are shown in a blue/white colony filter lift assay. GK and wild-type SH3GK were used as positive and negative controls, respectively, to determine GukH interaction (+) and no interaction (-). (b) CLUSTALW alignment of Dlg residues required for SH3GK repression with the corresponding regions of both MAGUK (R. norvegicus PSD-95, C. elegans LIN-2, H. sapiens MPP3) and non MAGUK (H. sapiens Fyn, and S. cerevisiae Guanylate Kinase) sequences. Amino acid identities are shaded and similarities are boxed. Secondary structure elements from the SH3 and GK domains of PSD-95 are shown above the sequences with β-sheets that fold into the SH3 domain as green arrows and α-helicies from the Hook region and GK domain as gray and orange cylinders respectively.