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. Author manuscript; available in PMC: 2013 Jan 3.
Published in final edited form as: Biochemistry. 2009 Oct 27;48(42):10014–10019. doi: 10.1021/bi901160f

Figure 4.

Figure 4

Mutations that allow SH3GK to bind GukH do not disrupt ‘E’-‘F’ strand interaction (a) Schematic representation of “E”-strand containing SH3 and “F”-strand containing GK domains used in GST pull-down assays. (b) GK domains with activating mutations interact with SH3. Western Blot to visualize His-tagged SH3 shows no difference in pull-down between wild-type GK and GK with an activating mutation. Pull-down of SH3 by immobilized GST-GK fusion protein. Lanes show input (20%) and protein bound by GST-alone control and GST-GK fusion proteins.