Figure 2.

Mean ± SD fold increase in gene expression of versican domains (A) and aggrecan (B) performed as described in Figure 1 in healthy and laminitic equine digital laminae for RNA extracted from 3 sections of dorsal lamina obtained from 8 healthy control horses (stippled bars), 6 horses at onset of Obel grade 1 lameness (diagonal-striped bars), and 5 horses at onset of Obel grade 3 lameness (crosshatched bars) and reverse transcribed into cDNA. *Value differs significantly (*P = 0.050; †P = 0.01), compared with the value for the control horses, as determined from 95% confidence intervals of the treated samples via a 1-way ANOVA.