Figure 8.

Photomicrographs of thin sections of laminae from a representative healthy horse (A), a representative horse at onset of Obel grade 1 lameness (B), and a representative horse at onset of Obel grade 3 lameness (C). Tissue sections were stained with an antibody against laminin (red) to enable evaluation of the basement membrane and phalloidin-FITC to enable evaluation of intracellular cortical actin (green). Nuclei (blue) were stained with DAPI. Notice the arrows marking detachment of basement membrane laminin from basal epithelial cells in the section of laminae collected at onset of Obel grade 3 lameness (C). Bar = 50 μm.