Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2013 Mar 3.

Published in final edited form as: J Vet Cardiol. 2012 Mar 3;14(1):211–221. doi: 10.1016/j.jvc.2012.02.006

Protein expression changes in canine MVIC with cyclic stretch and/or TGFβ1. (A) Immunofluorescent staining for alpha-smooth muscle actin (αSMA, red), vimentin (green), and DNA (blue) in MVIC cultured in 3D hydrogels. Cells were from 3D cultures that were under static control conditions (0,0), treatment with TGFβ1 alone (0,+), 15% cyclic strain alone (+,0) or both TGFβ1 and strain (+,+). Cells expressing αSMA (B) and vimentin (C) were quantified and normalized to total cell number.

Protein expression changes in canine MVIC with cyclic stretch and/or TGFβ1. (A) Immunofluorescent staining for alpha-smooth muscle actin (αSMA, red), vimentin (green), and DNA (blue) in MVIC cultured in 3D hydrogels. Cells were from 3D cultures that were under static control conditions (0,0), treatment with TGFβ1 alone (0,+), 15% cyclic strain alone (+,0) or both TGFβ1 and strain (+,+). Cells expressing αSMA (B) and vimentin (C) were quantified and normalized to total cell number.