Table 1.

Sexually Dimorphic Expression of CYP2C12 mRNA in Liver and Spleen-containing Hepatocyte Transplants.

Weeks Post
Transplantation	Recipient	Donor		CYP2C12/Albumin	% Liver CYP2C12
8	Male	Male	Liver	ND
			Spleen	ND	_____
		Female	Liver	ND	_____
			Spleen	ND	_____
	Female	Male	Liver	0.48±0.09
			Spleen	ND	_____
		Female	Liver	0.51±0.04
			Spleen	ND	_____
16	Male	Male	Liver	ND
			Spleen	ND	_____
		Female	Liver	ND
			Spleen	0.41±0.09a	2.2±0.4b
	Female	Male	Liver	0.63±0.10
			Spleen	0.87±0.27	3.6±1.2
		Female	Liver	0.78±0.11
			Spleen	0.89±0.24†	4.3±1.3†
30	Male	Male	Liver	ND
			Spleen	ND	_____
		Female	Liver	ND
			Spleen	0.13±0.04a	1.0±0.1b
	Female	Male	Liver	0.64±0.11
			Spleen	0.61±0.12	4.3±1.1
		Female	Liver	0.59±0.07
			Spleen	0.56±0.09†	4.1±1.0†
45	Male	Male	Liver	ND
			Spleen	ND	_____
		Female	Liver	ND
			Spleen	0.07±0.02a	0.9±0.2b
	Female	Male	Liver	0.73±0.11
			Spleen	0.61±0.12	12.6±3.8
		Female	Liver	0.76±0.17
			Spleen	0.63±0.13†	6.1±0.7†#

Donor hepatocytes isolated from adult male or female rats were infused into the spleens of same age male and female recipient rats. The recipients were euthanized at 8, 16, 30 and 45 weeks after transplantation when CYP2C12 mRNA levels were determined in both organs. Values are presented as CYP2C12 mRNA per rat albumin mRNA (liver and spleen) and as % of autologous liver CYP2C12 mRNA/mg (spleen). Each value is a mean ±SD with n=6 unless otherwise indicated. ND, not detectable,

^*, P<0.05 compares spleen with liver of the same rat.

^†, P<0.05 compares same sex donors in opposite sex recipients of the same age.

^#, P<0.05 compares opposite sex donors in same sex recipients of the same age.

^a, n=3,

^b, % of female liver.