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. Author manuscript; available in PMC: 2013 Jan 3.
Published in final edited form as: Am J Vet Res. 2012 Jul;73(7):1035–1046. doi: 10.2460/ajvr.73.7.1035

Figure 3. Immunoreactivity of ADAMTS-4 and its substrates in extracts of healthy equine laminae.

Figure 3

Western blots of laminar extracts (30 μg protein/lane) were probed using: A) antibody against neoepitope FASLSRFVET exposed on ADAMTS-4 catalytic domain after removal of the regulatory propeptide (NP-40 extract); B) antibody against versican V0/V1 neoeptiope DPEAAE generated by ADAMTS-4 cleavage (NP-40 extract); C) antibody against versican V0/V2 neoeptiope NIVSFE generated by ADAMTS-4 cleavage (NP-40 extract); D) antibody against aggrecan neoepitope ARGSVIL (BC-3) generated by ADAMTS-4 cleavage (guanidine hydrochloride extract digested with hyaluronidase, chondroitinase ABC and karatinase III).