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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: J Immunol. 2012 Dec 3;190(1):307–316. doi: 10.4049/jimmunol.1201644

Figure 3. Bacteria-induced cytokine gene expression is impaired in IRAK4KDKI macrophages.

Figure 3

(A) WT and IRAK4KDKI macrophages were treated with Pam3Cys (P3C, 1 μg/ml), LPS (100 ng/ml), heat-killed S. aureus (HKSA, 5 × 107 bacteria) or heat-killed P. aeruginosa (HKPA, 5 × 107 bacteria) for 1, 3 and 5 h. Samples were then analyzed for gene expression by qPCR. All samples were normalized to the housekeeping gene HPRT. Samples were tested in duplicate and each graph represents 3 separate experiments. (B) WT and IRAK4KDKI macrophages were treated with Pam3Cys (P3C, 1 μg/ml), LPS (100 ng/ml), or both for 3 h. Samples were then analyzed for gene expression by qPCR. (C) WT and IRAK4KDKI macrophages were treated with Pam3Cys (P3C, 1 μg/ml) or various concentrations of live S. pneumoniae (SP, MOI 1:1, 0.2:1 and 0.04:1). Samples were then analyzed for gene expression by qPCR. Each sample was normalized to HPRT and each graph represents 2 separate experiments.