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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: J Immunol. 2012 Dec 3;190(1):307–316. doi: 10.4049/jimmunol.1201644

Figure 5. Gene induction is impaired in the lungs of S. pneumoniae-infected IRAK4KDKI mice.

Figure 5

(A) WT or IRAK4KDKI mice were infected i.n. with 5 × 105 S. pneumoniae (SP) in a total volume of 30 μl or mock-infected with an equal volume of saline. After 48 h, mice were sacrificed, lungs were harvested, RNA was extracted and gene expression was measured by qPCR. Each sample was normalized to HPRT. Data shown is combined data from at least 3 separate experiments using a total of 4 mice (saline) or at least 9 mice (SP infected) per group. (*) indicates Student’s t test p ≤ .05; (NS) indicates no statistical difference (p > .05). (B, C) WT or IRAK4KDKI mice were infected as in (A). After 48 h, serum cytokines (B) or bacterial rRNA (C) was measured. Data is the combined data of 2 separate experiments using a total of 9 (B) or 6 (C) mice per group. (**) indicates Student’s t test p < .01.