Figure 4. In vitro digests of recombinant ICAM-5.

Recombinant mouse ICAM-5 was incubated with select MMPs as indicated, and the digestion products resolved by electrophoresis. Following transfer to a PVDF membrane, specific products were isolated for N terminal sequencing (arrowheads). Products denoted by the arrowheads in the MMP-3 and -9 digests in particular were analyzed (4a and b). Both MMPs generated a fragment having the construct’s original N terminus (uppermost arrowhead), as well as a fragment showing a new N terminus beginning with amino acid 780 (lowermost arrowhead).