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. Author manuscript; available in PMC: 2013 Jan 3.
Published in final edited form as: Biopolymers. 2011 Jan;95(1):17–23. doi: 10.1002/bip.21548

Figure 2. Isoxazolidine-driven gene expression requires CBP and the KIX domain.

Figure 2

HeLa cells were transfected with a plasmid encoding the DBD of Gal4 fused to the ligand-binding domain of the glucocorticoid receptor, a firefly luciferase reporter plasmid containing five Gal4 binding sites, and a Renilla transfection control. Additional plasmids were transfected as indicated for CBP, CBP shRNA, KIX, and E1A experiments. Four hours post-transfection, 10 μM 1a was added as a solution in DMSO such that the final concentration of DMSO was 1% (v/v). Luciferase output was measured 24h after addition of compound; fold activation is a ratio of firefly and Renilla luminescence divided by the ratio of firefly and Renilla signal observed with DBD alone. Each value represents at least three individual experiments with the indicated error (standard deviation of the mean, SDOM).