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RT-PCR analysis of MAdCAM-1 and GAPDH in SVEC exposed to TNF-α with or without DETA-NONOate. In controls, only a faint MAdCAM-1 transcript is detected (474 bp). MAdCAM-1 band is easily detected after TNF-α stimulation (20 ng/ml). DETA-NONOate (100 μM) blocked TNF-α-induced MAdCAM-1 transcript. In each panel, 307 bp bands indicate GAPDH transcripts, used to evaluate amount and quality of RNA. Similar results were obtained in 3 separate experiments.
