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Published in final edited form as: Immunobiology. 2012 Jul 27;218(4):561–569. doi: 10.1016/j.imbio.2012.07.022

Serum samples were obtained from each group of mice 1 week after the last injection of LPS or PBS. Analyses of (A) TNF-α (B) IL-2 (C) IFN-γ (D) MCP-1 (E) IL-4 (F) IL-12p70 (G) IL-6 and (H) IL-10 were performed with CBA as described in Materials and Methods. Values are mean ± SE (apoE-KO: PBS; n= 9, apoE-KO: LPS; n= 9; DKO: PBS; n= 10, DKO: LPS; n= 8). *p < 0.05 (I) IL-6 and TNF-α production by peritoneal macrophages of apoE-KO or DKO mice elicited with thioglycollate and stimulated with LPS (1 μg/ml, 0.1 μg/ml) in vitro for 24 hr. Cytokines in culture supernatant were quantified as described in Materials and Methods. N.S.: not significant. (J) Effect of co-culture with 2E10 NKT hybridoma cells on production of IL-6 and TNF-α by peritoneal macrophages upon stimulation with LPS. Peritoneal macrophages of apoE-KO or DKO mice were cultured in the presence of LPS (1 μg/ml) with 2E10 hybridoma cells or without hybridoma cells. **p < 0.01; N.S.: not significant. (K) Flow cytometric profiles of intracellular IFN-γ and IL-4 production by hepatic NKT cells 2 hr after α-GalCer, LPS or PBS injection into WT mice. Numbers indicate the percentage of cytokine-positive NKT cells (Δ percentage of isotype control). Representative profile of 3 independent experiments. (L) The intracellular IFN-γ and IL-4 production profile of hepatic NK1.1⁺TCRβ⁺ NKT cells in WT mice. Data shown indicate the mean percentage ± SE of cytokine-positive NKT cells from 3 independent experiments.

Serum samples were obtained from each group of mice 1 week after the last injection of LPS or PBS. Analyses of (A) TNF-α (B) IL-2 (C) IFN-γ (D) MCP-1 (E) IL-4 (F) IL-12p70 (G) IL-6 and (H) IL-10 were performed with CBA as described in Materials and Methods. Values are mean ± SE (apoE-KO: PBS; n= 9, apoE-KO: LPS; n= 9; DKO: PBS; n= 10, DKO: LPS; n= 8). *p < 0.05 (I) IL-6 and TNF-α production by peritoneal macrophages of apoE-KO or DKO mice elicited with thioglycollate and stimulated with LPS (1 μg/ml, 0.1 μg/ml) in vitro for 24 hr. Cytokines in culture supernatant were quantified as described in Materials and Methods. N.S.: not significant. (J) Effect of co-culture with 2E10 NKT hybridoma cells on production of IL-6 and TNF-α by peritoneal macrophages upon stimulation with LPS. Peritoneal macrophages of apoE-KO or DKO mice were cultured in the presence of LPS (1 μg/ml) with 2E10 hybridoma cells or without hybridoma cells. **p < 0.01; N.S.: not significant. (K) Flow cytometric profiles of intracellular IFN-γ and IL-4 production by hepatic NKT cells 2 hr after α-GalCer, LPS or PBS injection into WT mice. Numbers indicate the percentage of cytokine-positive NKT cells (Δ percentage of isotype control). Representative profile of 3 independent experiments. (L) The intracellular IFN-γ and IL-4 production profile of hepatic NK1.1⁺TCRβ⁺ NKT cells in WT mice. Data shown indicate the mean percentage ± SE of cytokine-positive NKT cells from 3 independent experiments.