Figure 5. Flow cytometric analysis of liver NK cells and atherosclerotic lesion areas in apoE-KO mice depleted of NK cells and treated with LPS.

(A) Numbers of NK cells in liver from apoE-KO or DKO treated with LPS or PBS were calculated from flow cytometric data and the actual cell counts. (B) Mean number of NK cells in liver from apoE-KO mice depleted of NK cells with rabbit anti-asialoGM1 Ab as described in Materials and Methods. Numbers of NK cells in liver were calculated from flow cytometric data and the actual cell counts (NK depletion: PBS; n= 5, NK depletion: LPS; n= 5). (C) Mean lesion areas of apoE-KO mice depleted of NK cells (NK depletion) and administered LPS or PBS. Each symbol represents the lesion area of an individual mouse. Horizontal bars represent the mean of all mice in each group, and vertical bars represent SE. * p < 0.05