Fig 2.

Reduced cell invasiveness in filamin-A deficient cells. Boyden chamber trans-well assay was used to assess the cell invasiveness. Serum deprived cells were seeded in cell culture inner chamber coated with Matrigel, and complete growth medium was added into outer chamber as chemoattractant. Panel A shows the numbers of cells migrated from the inner chamber side of the membrane to outer chamber side of the membrane after 4, 12 and 24 hours incubation. The insertions in panel A are showing the expression levels of filamin-A in paired cell lines detected by Western-Blot. β-actin was used as loading control. Panel B shows the DAPI staining of cells attached to outer side of the membrane after 24 hours incubation (*p<0.05, and ** p<0.01).