Fig 3.

Effect of filamin-A on organ metastasis in nude mouse xenograft model. Tumor bearing mouse was generated by injecting EGFP-expressing C8161-con or C8161-KD melanoma cells into nude mice subcutaneously. Mice were sacrificed to collect lungs and spleens at 5-week after the inoculation. Panel A shows the lung metastases after Bouin's solution staining (Red arrows). Panel B shows the IHC staining with GFP antibody and HE staining of lung tissue section from mouse with C8161-con tumor, which indicates the lung metastasis focus. Panel C shows the image of representative spleens collected from non-injecting mice, C8161-con tumor bearing mice, and C8161-KD tumor bearing mice. Panel D shows the average weight of spleens from three groups of mice.