Table 2.

Summary of microscopic rates determined for SR-95531 and GABA

	KD-SR (µM)	koff-SR (s−1)	kon-SR (M−1s−1)	kon-GABA (M−1s−1)
Wild-type	0.14	15.9 ± 0.8	(1.14 ± 0.10) × 108	(7.36 ± 0.42) × 106
Y97A	7.68	832.1 ± 91.5 *	(1.08 ± 0.12) × 108	(2.17 ± 0.13) × 106 *
Y97F	1.67	188.9 ± 9.7*	(1.13 ± 0.06) × 108	(5.66 ± 0.62) × 106 †
Y157F	0.26	44.4 ± 1.6 *	(1.71 ± 0.06) × 108	(1.60 ± 0.31) × 106 *
Y205F	0.87	121.0 ± 7.9 *	(1.39 ± 0.09) × 108	(6.03 ± 0.44) × 105 *

Significant differences between wild-type and mutant receptors were calculated using ANOVA with a Dunnett’s post-test

^*p < 0.01;

^†p < 0.05