Table 3.
Detection of Nucleic Acids by PNAsa
| Nucleic Acid Type | Lower Limit of Qualititative Detection | Lower Limit of Quantitative Detectionb | |||
|---|---|---|---|---|---|
| Name | Template (SP1-RP1) | Crosslink (SP3-RP3) | Template (SP1-RP1) | Crosslink (SP3-RP3) | |
| TS1 | DNA (27bp) | 6.02 × 103 | 4.15 × 106 | 1.13 × 108 | 6.62 × 108 |
| (1.09 × 108)c | |||||
| TS2 | DNA (27bp) | 6.02 × 103 | 4.15 × 106 | 2.53 × 108 | 9.03 × 108 |
| (ds) | (2.19 × 107)c | ||||
| TS3 | DNA (50bp) (ds) | 2.14 × 107 | x | 4.49 × 109 | x |
| TC mismatch | DNA (27bp) | 6.02 × 103 | x | (1.86 × 108)c | x |
| TG mismatch | DNA (27bp) | 6.02 × 103 | x | (2.66 × 108)c | x |
| TT mismatch | DNA (27bp) | 6.02 × 103 | x | (1.60 × 108)c | x |
a
Unless otherwise noted, values were determined by curve fits to VMax (mAbs652 nm*min-1) vs. [DNA] data.
b
Values for quantitative detection are the number of molecules detected at 90% CI and 96% minimal distinguishable difference concentration.
c
Values determine from curve fit to absorbance at 450 nm vs. [DNA].