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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Biol Cell. 2012 Dec 5;105(1):1–13. doi: 10.1111/boc.201200044

Figure 5. A cryo-EM image of microtubules decorated with dimeric Ncd at subsaturating ratios reveals a cooperative binding mechanism in the axial direction, along individual protofilaments.

Figure 5

While one of the microtubules is mostly decorated (I), partial decoration (III) and complete decoration (II) co-exist in the same area. A Fourier-filtered image of the microtubule and the motors in a side projection allows determination of the polarity of the microtubule according to the shape of the dimeric motor construct (Adapted from Figure 1 of (Wendt et al., 2002)).