Effect of yeast extract on the survival of mycoplasmas that are bound to MH-S cells. Panels (a) to (c), survival of mycoplasma strains CTG1701-C, CTG1701, and CTG38, respectively, grown in assay buffer with and without yeast extract. Panel (d), comparative survival of CTG1701-C, CTG1701, and CTG38, bound to MH-S cells and incubated in assay buffer with yeast extract. In all panels, the abbreviation YE in the strain designations indicates that yeast extract was added to the assay buffer. Significant differences are indicated by an asterisk. Panel (a), the survival of CTG1701-C is significantly higher at 8 hours when yeast extract is absent (P < 0.001). Panel (b), the survival of CTG1701 is significantly higher at 4 and 8 hours (P < 0.001 and 0.002, respectively) when yeast extract is absent. Panel (c), the survival of CTG38 is significantly higher at 4 and 8 hours (P = 0.015 and 0.002, respectively) when yeast extract is absent. Panel (d), the survival of CTG1701-C was significantly higher than that of CTG1701 and CTG38 at 8 hours (P < 0.001 for both strains). Error bars represent standard error of the mean and in some cases are too small to visualize on the graphs. The results were replicated with n values of 6 for the data in all panels.