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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: J Mol Cell Cardiol. 2012 Oct 30;54:1–8. doi: 10.1016/j.yjmcc.2012.10.010

Figure 2.

Figure 2

Normalized tension-pCa relationships without and with alkaline phosphatase (AP) treatment. A. Thin filament calcium sensitivity was reduced due to PTU diet in myocardial strips not treated with AP. B. After AP treatment, thin filament calcium sensitivity was normalized to that of nonPTU-fed mice. The remainder of this study focused on AP treated myofilaments. C. AP treatment was found to reduce phosphorylation of cardiac troponin-I (cTnI) at Serines-23,24 in both normal and hypothyroid SvEv mice. Ponceau stain is used to detect total protein content on nitrocellulose prior to Western blot. D. Phosphorylation of cardiac regulatory light chain (MLC2) at Serine-19 appeared to be reduced by BDM exposure in the skinned strips compared to homogenate (hom) [51] and reduced further by AP treatment.