. Author manuscript; available in PMC: 2014 Jan 1.

Published in final edited form as: J Mol Cell Cardiol. 2012 Oct 30;54:1–8. doi: 10.1016/j.yjmcc.2012.10.010

Table 2.

Variables of myosin enzyme kinetics model applied to myosin detachment rate 2πc vs MgATP relationships (Equation 3).

	WKY (n=4) 100% α	WKY_PTU (4) 100% β	SvEv (7) 100% α	SvEv_PTU (6) 100% β	FVB (8) 100% α	FVB_βTG (4) 70% β-30% α	predicted100% β
k_−ADP (s⁻¹)	65.0±7.9	15.5±0.8^*	111.4±6.2	24.3±1.8^†	115.1±5.1	48.9±3.1^†	20.5
k_+ATP (mM⁻¹.s⁻¹)	108±10	55±6^*	325±32	152±23^†	338±41	212±31^*	158
t_−ADP (ms)	18.2±1.9	64.9±3.3^†	9.1±0.4	42.3±2.9^†	9.3±0.4	21.3±1.6^†	26.4 (48.7)
[MgATP]₅₀ (μM)	579±66	334±14^*	365±43	178±25^†	394±49	271±31^#	218

Mean time period of MgADP state, t_−ADP = 1/ k_−ADP, and MgATP concentration at half maximum detachment rate, [MgATP]₅₀ = k_−ADP/k_+ATP, are also provided.

^†

P<0.01,

P<0.05,

P<0.1 against 100% α-MyHC controls. Predicted values for 100% β-MyHC in the FVB strain were calculated as (FVB_βTG – 0.3 × FVB) / 0.7; an alternative value for the predicted t_−ADP, given in parentheses, is the reciprocal of the predicted k_−ADP.