(A) Detection of crispld2 mRNA by RTPCR during early development (NTC = no template control, UWT = unfertilized wild type embryo, dpf = days post fertilization, hpf = hrs post fertilization). (B-H) in situ hybridization showing crispld2 expression (purple) at the following stages: (B) 5-7 somite, (C) 13-15 somite, (D) 1 dpf, (E) 2 dpf, (F) 3 dpf, (G) 4 dpf, and (H) 5 dpf.