Methyl TROSY experiments on Hrp1 protein. Results obtained for Hrp1–RBD (A), Hrp1–RBDCTD (B), and the Hrp1-full length proteins (C) are shown. (Top) The structure of each segment with Ile, Leu, and Val residues highlighted with pink spheres. The Hrp1–RBD structure was taken from the 2CJK and 2KM8 structures, whereas Hrp1–RBDCTD and Hrp1-full length were modeled using I-TASSER. (Middle) 1H-13C HMQCs of Hrp1–RBD, Hrp1–RBDCTD, and Hrp1-full length proteins. (Bottom) 1H-15N HSQCs for the same constructs.