Skip to main content
. 2012 Dec 10;109(52):21342–21347. doi: 10.1073/pnas.1214102110

Fig. 3.

Fig. 3.

Interaction of Hrp1 with the Rna14–Rna15 tetramer by pull-down experiments and NMR chemical shift perturbation analysis. (A) GST Pull-down of Hrp1 (full length; FL) and Hrp1 (RBD) with His-tagged Rna14–Rna15. Lanes 1 and 6 are Hrp1 FL and Hrp1 RBD with Rna14–Rna15 in the absence of RNA. Lanes 2–5 are Hrp1 FL incubated with Rna14–Rna15 with different amounts of RNA, 7–10 are Hrp1 RBD and Rna14–Rna15 with different amounts of RNA. (B) Chemical shift perturbation of Hrp1 upon addition of RNA and of the Rna14–Rna15 complex. Hrp1 RBD (free) is represented in red, the RNA-bound complex is shown in blue, and the Rna14–Rna15 bound complex is shown in green. A cartoon model summarizing the chemical shift perturbation results is shown at the top.