Fig. 4.

Model of the CF I complex. The Hrp1, 3′-end processing substrate RNA and Rna14–Rna15 complexes were used in HADDOCK to model the organization and interaction of components of the complete CF I complex. Starting from the complex of Hrp1 with RNA, the Rna14–Rna15 complex was added to generate a model of the complete CF I complex. Here, Hrp1 is shown in magenta, Rna15 proteins are shown in green-chartreuse and green-forest colors, whereas Rna14 monomers are shown in cyan and olive colors and the RNA is depicted by its secondary structure. The residues of Hrp1 interacting with Rna14 are shown with green spheres. Images were generated with Pymol (www.pymol.org).