. 2012 Dec 10;109(52):21295–21300. doi: 10.1073/pnas.1211762109

Table 1.

K_i and K_d values for binding of htMVL 1 and htMVL 2 (bivalent vs. monovalent)

Compound	MC4R cells		Dual Express		Fold increase^‡	CCK2R cells		Dual Express		Fold increase^‡
Compound	K_i or K_d nM	n^¶	K_i or K_d nM	n	Fold increase^‡	K_i or K_d nM	n	K_i or K_d nM	n	Fold increase^‡
htMVL 1^*	353.6 ± 97.1	4	14.6 ± 1.4	4	24.2^§	237.5 ± 26.5	4	88.9 ± 9.2	4	2.7^§
htMVL 2^†	381.4 ± 15.0	3	16.7 ± 2.0	4	22.9^§	31.3 ± 1.2	3	16.7 ± 2.0	4	1.9^§

*htMVL1 is expressed as K_i value. For MC4R receptors, K_i values were calculated with the equation K_i = IC₅₀/(1+([ligand]/K_d), where IC₅₀ values from competition binding assays; [ligand] = 10 nM Eu-NDP-α-MSH; K_d = 8.3 nM. For CCK2R receptor, where [ligand] = 1 nM Eu-CCK; K_d = 35 nM.

^†htMVL 2 is expressed as K_d value from saturation binding assay.

^‡Ratio of monovalent (MC4R or CCK2R cells) to bivalent binding (dual receptor expressing cells).

^§P < 0.05 (MC4R or CCK2R cells vs. dual receptor expressing cells).

^¶n refers to the number of independent binding experiments.