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. 2012 Nov 14;109(52):21307–21312. doi: 10.1073/pnas.1215689109

Fig. 3.

Fig. 3.

The cut gene cluster is responsible for choline metabolism and TMA production. (A) Growth of D. alaskensis G20 wild-type and C10(pB6) strains at 37 °C on lactate sulfate (black), choline sulfate (red), and choline fermentation (blue) media. The data shown are the average OD600 values of four cultures. Error bars represent SEM. (B) LC-MS quantification of d9-TMA produced by D. alaskensis G20 wild type and C10(pB6) during incubations in (trimethyl-d9)-choline sulfate and (trimethyl-d9)-choline fermentation media containing (trimethyl-d9)-choline chloride (60 mM). Bar graphs represent the mean ± SEM of four cultures. *P < 0.02; **P < 10−5; ***P < 10−6. (C) LC-MS quantification of d9-TMA produced by E. coli BL21(DE3) during incubations in Luria–Bertani (LB) medium containing (trimethyl-d9)-choline chloride (100 µM). Bar graphs represent the mean ± SEM of four cultures. **P < 10−5.