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. 2012 Dec 12;109(52):21528–21533. doi: 10.1073/pnas.1215849110

Fig. 3.

Fig. 3.

Loss of intestinal integrity, AMP expression, and IIS in aging flies. (A) Systemic expression of drosomycin (Drs), drosocin (Dro), and diptericin (Dpt) in 10-, 25-, and 40-d-old non-Smurf (SMF−) and Smurf (SMF+) w1118 flies. At 10 d, n = 3 female flies, 6 replicates; at 25 and 40 d, n = 3 female flies, 8 replicates. Increases in Dro and Dpt expression between 10 and 40 d in the SMF− population are also statistically significant (P values: Dro, <0.05; Dpt, <0.05). (B) Total number of bacterial colony-forming units (CFU) cultured from the interior of 25-d-old non-Smurf (SMF−) and Smurf (SMF+) w1118 flies. n = 3 female flies, 5 replicates. (C) Systemic expression of Thor, InR, and ImpL2 in 10-, 25-, and 40-d-old non-Smurf (SMF−) and Smurf (SMF+) w1118 flies. At 10 d, n = 3 female flies, 6 replicates; at 25 and 40 d, n = 3 female flies, 8 replicates. Expression levels of Thor and ImpL2 are significantly reduced in the SMF− population at 25 d (P values: Thor, <0.01; ImpL2, <0.01) relative to 10 d. Thor is also reduced at 40 d (P < 0.01). InR shows no significant difference in expression in the SMF− population. (D) Ratio of phosphorylated Akt level to total Akt level in 25-d-old non-Smurf (SMF−) and Smurf (SMF+) w1118 flies, calculated after normalization to total protein loaded per lane. n = 3 female flies, 3 replicates. Error bars represent mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.