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. 2012 Dec 10;109(52):E3687–E3695. doi: 10.1073/pnas.1209990110

Fig. 2.

Fig. 2.

Differential regulation of AChE exons after FSS and drug treatment. (A) Schematic representation of the course of the experiment. After the FSS protocol had been followed for 4 d, the mice were returned to their original cages for 1 wk and then were injected with NaBu, TSA, or saline daily for 1 wk and were killed 2 wk thereafter (n = 4). (B) Semiquantitative RT-PCR analysis of the AChE 5′ transcripts (mE1a–mE1e) in the hippocampus of control or stressed mice treated with saline or an HDACi (NaBu or TSA). Lane 1, saline; lane 2, stress + saline; lane 3, stress + NaBu; lane 4, stress + TSA. NaBu completely restored the down-regulation of mE1b and mE1c exons at the 5′ end. TSA, on the other hand, showed variable effects. (C–E) Real time PCR of mE1c (C), AChE-R (D), and AChE-S (E) in the hippocampus of control mice, stressed mice, and stressed mice treated with either saline or NaBu. At the 3' end, similar restoration was found in AChE-R mRNA levels, while AChE-S mRNA levels were partially restored with NaBu treatment. *P < 0.05, 2-tailed u test.