Fig. 3.

Biological analysis of the renal cystic index in various Gpsm1;Pkd1 genotyped mice. Mouse kidneys were harvested between postnatal days 11 and 12 from noncystic Pkd1^+/+ and Pkd1^+/V and cystic Pkd1^V/V mice with various Gpsm1 genotypes (i.e., Gpsm1^+/+, Gpsm1^+/−, or Gpsm1^−/−). (A) Representative whole kidneys were photographed to demonstrate the differences in gross morphology among the various genotyped mice. (B) KW/BW ratios were calculated and graphed as a percentage. Numbers analyzed for each group with a distinct genotype are provided in the graph. *P < 0.05: significant difference between Gpsm1^+/+;Pkd1^V/V and Gpsm1^+/−;Pkd1^V/V versus Gpsm1^−/−;Pkd1^V/V. ^¶P < 0.001: significant difference between the Pkd1^V/V versus Pkd1^+/+ and Pkd1^+/− mouse kidneys. (C) Kidneys obtained from mice at postnatal days 11–12 as shown in A were sectioned and stained with H&E. Four representative whole-kidney sections are shown from the three different Gpsm1 genotyped mouse strains on the Pkd1^V/V background. (Scale bar: 2.98 mm.) Kidney sections were morphometrically analyzed as described in Materials and Methods and graphed in D to determine the surface area of the remaining kidney tissue relative to the tubular (cyst) space. *P < 0.05: significant difference between Gpsm1^+/+;Pkd1^V/V and Gpsm1^+/−;Pkd1^V/V versus Gpsm1^−/−;Pkd1^V/V mouse kidneys.