Fig. 4.

Anterograde axonal transport of SCG10. (A) Live imaging of Venus-tagged SCG10 in cultured DRG axons. (Upper) Four single frames with the same time interval are shown as representative images. Arrowhead indicates tracking of ven-SCG10 puncta. (Lower) The axon is visualized by maximum projection of all single frames in the time-lapse images. (Scale bar, 10 µm.) (B) A kymograph generated from the axon shown in A demonstrates that ven-SCG10 is transported predominantly in the anterograde direction. The kymograph corresponds to a 79-s live imaging. d, distance; t, time.